HeMoStep Kit:

The first tool to successfully detect and quantify blood contamination in cerebrospinal fluid

High sensitivity, accuracy, and reliability in just 55 minutes.

Welcome to the future of CSF testing! We are thrilled to introduce our groundbreaking method that will revolutionize how you DETECT and QUANTIFY blood contamination in cerebrospinal fluid samples.

With relative frequency, lumbar puncture procedure is complicated by the contamination of CSF with blood cells as result of needle trauma, also known as traumatic tap.

No more confusing diagnoses and analysis complications due to the presence of blood in Cerebrospinal Fluid samples thanks to the HemoStep Kit.

Quantifying blood contamination in CSF by Flow Cytometry

Current methods for the determination of contamination in CSF consist of applying corrective formulas based on the assumption that the ratio of WBCs to RBCs in CSF will be identical to that in blood.

This determination is only correct if neither WBC nor red blood cells are lysed in the CSF during the time between collection of the liquid and the cell count, which is very unusual.

New high-performance method for evaluation of CSF contamination by Flow Cytometry

Quantitative assay measuring blood contamination in CSF

Data Analysis to extract and interpret assay results


Our method delivers results with exceptional sensitivity, ensuring you can detect even the slightest traces of blood contamination with precision.

Experience high specificity without known interferents, providing you with confidence in your CSF testing.

HeMoStep seamlessly works with stabilized samples, enhancing the flexibility of your workflow.

Say goodbye to cytotoxic effects and cell losses. Our solution is designed to safeguard your samples.

Streamline your testing process with a protocol that takes just 55 minutes, saving you valuable time without compromising accuracy.

Count on reliable and reproducible results every time you use our method, ensuring consistent outcomes for your research or clinical applications.

Principle of Method

A total of 115 CSF lysis supernatants from patients with infiltrating leukemia’s and lymphomas, of which 34 showed traumatic punctures, were analyzed by FCM for peripheral blood contamination comparing both classical neutrophil count vs. the new method with HeMoStep kit.

The new method is based on a sandwich bead-based assay that enable the HbA0 quantification and correlated with the degree of contamination in RBC/µl.

Improved method by flow cytometry

The developed method allows the determination CSF peripheral blood contamination in a <1h protocol circumventing the problems arising from CSF cytotoxicity and minimizes sample usage, which is particularly important in paucicellular samples, by using as sample the supernatant of RBC lysis that is usually discarded.

Quantification of  visibly undetectable contaminations

High analytical sensitivity – LoD: [3,15 ng/ml]

The analytical sensitivity of the kit allows it to detect contaminations lower that 1 RBC/µ.

Expected values of contamination in [RBC/µ] (n=115).

Correct classification between traumatic and non-traumatic LP samples with great accuracy

Expected values of contamination in [HbA0 ng/ml] in traumatic lumbar puncture (n=34).

Neutrophil absolute counting method detected contamination in 70% of the samples identified as traumatic punctures, while the new method detected peripheral blood contamination in 100% of these samples.

Agreement between methods

High positive linear correlation (r=0.9) with respect to other methods, like granulocyte or RBC count (n=115).

Compatible with stabilized samples

Comparative between same sample stabilized with Transfix (1:20) and without stabilization (fresh).

What this kit includes?

Capture Beads

5ml of Capture Beads coated with an Hb-specific monoclonal antibody.

Wash Buffer

25ml wash buffer (10X).

Control +

Positive control. 5 vials of lyophilised RBCs lysate.

Calibration Beads

1ml of calibration beads coated with two different concentrations of Hb.



Standard of known concentration. 1 vial of lyophilised RBCs lysate with known [Hb] concentrations.

Detector Antibody

1,2 ml fluorescently conjugated (PE) detector antibody (10μl/test).

How to interpolate values obtained with HeMoStep kit

In this video you can see how easy is to use GraphPad to interpolate the values of the test performed with HeMoStep kit.

Take into consideration that the version of GraphPad used is 8.0, so the user interface could vary depending on the version, but the procedure will be very similar.

If there is still any question about this procedure in particular or about other , our team of specialists are available to answer, just complete the form below and we will reply with all the information needed.

Product nameReferenceDescription
HeMoStep Kit

Immunobead assay for quantitatively measure of blood contamination in CSF

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Frequently Asked Questions (FAQs)

Can be HeMoStep kit used with stabilised samples?

Yes, samples have been tested with both Transfix and Streck compared to the unstabilised sample. Neither of these interferes, so stabilised samples can be used with confidence for the HeMoStep kit.

Does HeMoStep kit detect total haemoglobin?

Yes, the HeMoStep kit is designed to detect and quantify total haemoglobin.

Can the procedure for lysis of RBCs affect the number of leukocytes present in the sample?

No, the number of leukocytes is not affected and it is recommended to lyse all samples, according to the EUROFLOW guidelines, including those CSF samples in which there is no visible contamination with RBCs. In this sense, no significant differences are observed between the lysis step (FACS Lysing) and the PBS washing step, being the manipulation of the sample, and in particular the decanting of the supernatant, and not the lysis, the main cause of cell loss, so it is recommended to aspirate and not decant the supernatants after the centrifugation steps.