HeMoStep Kit:
The first tool to successfully detect and quantify blood contamination in cerebrospinal fluid
High sensitivity, accuracy, and reliability in just 55 minutes.
Welcome to the future of CSF testing! We are thrilled to introduce our groundbreaking method that will revolutionize how you DETECT and QUANTIFY blood contamination in cerebrospinal fluid samples.
With relative frequency, lumbar puncture procedure is complicated by the contamination of CSF with blood cells as result of needle trauma, also known as traumatic tap.
No more confusing diagnoses and analysis complications due to the presence of blood in Cerebrospinal Fluid samples thanks to the HemoStep Kit.
New high-performance method for evaluation of CSF contamination by Flow Cytometry
Data Analysis to extract and interpret assay results
Advantages
Principle of Method
A total of 115 CSF lysis supernatants from patients with infiltrating leukemia’s and lymphomas, of which 34 showed traumatic punctures, were analyzed by FCM for peripheral blood contamination comparing both classical neutrophil count vs. the new method with HeMoStep kit.
The new method is based on a sandwich bead-based assay that enable the HbA0 quantification and correlated with the degree of contamination in RBC/µl.
Improved method by flow cytometry
The developed method allows the determination CSF peripheral blood contamination in a <1h protocol circumventing the problems arising from CSF cytotoxicity and minimizes sample usage, which is particularly important in paucicellular samples, by using as sample the supernatant of RBC lysis that is usually discarded.
Quantification of visibly undetectable contaminations
High analytical sensitivity – LoD: [3,15 ng/ml]
The analytical sensitivity of the kit allows it to detect contaminations lower that 1 RBC/µ.
Expected values of contamination in [RBC/µ] (n=115).
Correct classification between traumatic and non-traumatic LP samples with great accuracy
Expected values of contamination in [HbA0 ng/ml] in traumatic lumbar puncture (n=34).
Agreement between methods
High positive linear correlation (r=0.9) with respect to other methods, like granulocyte or RBC count (n=115).
Compatible with stabilized samples
Comparative between same sample stabilized with Transfix (1:20) and without stabilization (fresh).
What this kit includes?
Capture Beads
5ml of Capture Beads coated with an Hb-specific monoclonal antibody.
Wash Buffer
25ml wash buffer (10X).
Control +
Positive control. 5 vials of lyophilised RBCs lysate.
Calibration Beads
1ml of calibration beads coated with two different concentrations of Hb.
Standard
Standard of known concentration. 1 vial of lyophilised RBCs lysate with known [Hb] concentrations.
Detector Antibody
1,2 ml fluorescently conjugated (PE) detector antibody (10μl/test).
