ExoStep Platform: Detection and Characterization of Exosomes

ExoStepTM KIT: The most accurate method of exosome detection developed to date.

ExoStepTM kit is the most accurate method of exosome isolation developed to date, a superior alternative for the sensitive detection of exosomes compared with the most commonly used methods. Our kit is easy to implement and analyse for any laboratory that has access to a conventional flow cytometer.

Specific Exosome Detection in biological fluids by Flow Cytometry

Wide dynamic range and limit of detection

Allowing simultaneous Immunophenotyping of captured exosomes population

Easy to perform Immunobead assay with quantitative and scalable results

Specific and unambiguous exosome detection in biological fluids by flow cytometry:

ExoStepTM kit is an Immunobead assay for the detection of exosomes using a bead-bound capture antibody and fluorochrome conjugated detection antibody. This kit provides reproducible results and is intended for Flow Cytometry analysis of pre-enriched human exosomes from biofluids (plasma, serum, urine, among others) or cell culture media.

Ultracentrifugation is not needed

Reproducible results

Effective with small sample quantities

We are looking forward to hearing about your research

 Bead-based assay for Flow Cytometry

ExoStepTM  Kit for the Detection and Characterization of exosomes by Flow Cytometry is composed of an exosome capture reagent consisting of 6 µm diameter magnetic beads coated with a specific antibody as solid suport detectable by the cytometer. Additionally, the capture beads are dyed red and fluorescently labelled which facilitate their detection in the fluorescent detector of a conventional flow cytometer. ExoStepTM contains a detector antibody fluorescent conjugated for exosome detection.

*ExoELISA step Kit: ELISA Format Now Available

Wide dynamic range and limit of detection

To determine the dynamic range and limit of detection of the assay, EVs titration experiments were performed using CD63 for capture and CD9 for detection. The sensitivity of the assay has demonstrated to be very high with a positive signal detected as little as thirty ng of exosomes while 2 ug were required for Western Blot detection. Besides, Flow Cytometry data follows a linear distribution between 30 and 8 micrograms of exosomes, allowing interpolating fluorescent intensity values to estimate the amount of exosomes in the sample.

Video Protocol

We work to apply our technology in the detection and quantification of disease biomarkers present in the surface of EVs as well as in diagnostic of diseases.

*This Technology is under patent licence: PET/EP2021/068633 “Method for the detection and/or quantification of extracellular vesicles in fluid biological samples”

Simultaneous Immunophenotyping 

Considering exosome direct detection results in body fluids, we want to anticipate researcher’s needs for exosome subpopulations characterization through specific markers. In order to optimize the protocol, we performed an Immunophenotyping assay simultaneously with the exosome detection. This optimization of the protocol made possible to include in the FL2 channel (the most sensitive), the antibody against the marker of interest. The suitability of the kit for the specific detection of exosomes was confirmed, making possible to further characterize their phenotype by labelling with other markers of interest

ExoELISA-Step Kit

ExoELISA-Step is an enzyme immunoassay for the detection and quantification of exosomes, based on the use of antigens or antibodies labeled with an enzyme, so that the resulting conjugates have both immunological and enzymatic activity. As one of the components (antigen or antibody) is labeled with an enzyme and insolubilized on a support (immunosorbent), the antigen-antibody reaction will remain immobilized and therefore, is easily revealed by the addition of a specific substrate. When acting on the enzyme a simple or quantifiable view will produce an observable color using a spectrophotometer or a colorimeter.

You might be also interested in:

ExoStep Mouse

 Monoclonal antibody intended for mouse exosome detection using flow cytometry included.

Size Exclusion Columns (SEC) 

Combine our Size Exclusion Columns (SEC) with ExoStepTM to isolate and detect exosomes in small samples.

Detection Antibodies

Monoclonal antibody intended for the exosome detection using flow cytometry.


    References

    Kit NameKit Description+info
    ExoELISA-Step Cell Culture

    96 test | EXO2506 | Cell culture Samples | ELISA Assays for detection and quantification of exosomes |

    Download TDS

    Go to shop
    ExoELISA-Step Serum/Plasma

    96 test | Exo2508 | Serum/plasma Samples | ELISA Assays for detection and quantification of exosomes |

    Download TDS

    Go to shop
    ExoStep Culture

    25 test | ExoS-25-C9 | Cell culture Samples | Superparamagnetic Capture Beads (CD63), Primary detection antibody (CD9 PE) and Assay Buffer 10X |

    Download TDS

    Go to shop
    ExoStep Serum/Plasma

    25 test | ExoS-25-P81 | Serum/Plasma Samples | Superparamagnetic Capture Beads (CD9 Capture Beads) Primary detection antibody (CD81 PE) Assay Buffer 10X |

    Download TDS

    Go to shop
    ExoStep Urine

    25 test | ExoS-25-U9 | Urine Samples | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 PE) Assay Buffer 10X |

    Download TDS

    Go to shop
    ExoStep Culture + Standard

    50 test | ExoS-50-CST9 | Cell culture Samples | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 PE) Assay Buffer 10X Lyophilized exosomes (1×10^12) from PC-3 Human prostate cancer |

    Download TDS

    Go to shop
    ExoStep Serum/Plasma + Standard

    50 test | ExoS-50-PST81 | Serum/Plasma Samples | Superparamagnetic Capture Beads (CD9 Capture Beads) Primary detection antibody (CD81 PE) Assay Buffer 10X Lyophilized exosomes (1×10^12) from Human Plasma |

    Download TDS

    Go to shop
    ExoStep General Kit

    50 test ● 25 test | ExoS-50-G9 ExoS-50-G81 ExoS-25-G9 ExoS-25-G81 | Any biological Sample | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 Biotin) or (CD81 Biotin) Secondary detection reagent (PE Conjugated) Assay Buffer 10X |

    Download TDS

    Go to shop
    Mouse ExoStep Culture

    25 test | MO2ExoS-25-C9 | RUO mouse exosome from Cell Culture Samples | Superparamagnetic Capture Beads (CD63 Capture Beads) Primary detection antibody (CD9 Biotin) Secondary detection reagent (PE Conjugated) Assay Buffer 10X |

    Download TDS

    Go to shop