The most suitable method to isolate exosomes from biological samples
Size Exclusion Chromatography (SEC) has been described as the most efficient method for isolating EVs from complex biological fluids in one single step, with a good recovery and with almost complete removal of contaminants, such as proteins and lipoproteins. At Immunostep, we have developed 70nm and 35nm SEC columns for EVs isolation from fluids such as: plasma, serum, urine and cerebrospinal fluid.
Fast & reliable exosome purification
Reusable columns and efficient packaging
Successfully separating different EVs sizes
Great performance separating exosomes from important contaminants:
Immunostep reusable Size Exclusion Columns enable the rapid isolation of extracellular vesicles (EVs) from cell culture supernatants and complex biological fluids. Each column efficiently removes background proteins, lipids, solutes, and other contaminants to improve the sensitivity and accuracy of downstream assays (e.g., TRPS, protein profiling, RNA profiling, etc.) while maintaining the biological properties of the EVs. Vesicles are isolated in 15 minutes allowing samples to be collected and analysed immediately. Columns are available off-the-shelf, and quality assured, so your isolations are reproducible from column to column.
Figure 1: (A) Amount of EVs and protein in each fraction from the column. Comparative of protein (BCA) vs vesicles (FACS CD63+/CD9+) content. (B) Flow cytometric Analysis of elution fractions. Stain Index = (MFI Positive-MFI background 2σ background) (C) Western Blot SEC fractions were loaded on SD-PAGE and immunoblotted for CD9 tetraspanin with anti-CD9 (VJ1/20), under no-reducing conditions.
Successfully separating different EVs sizes
EVS SEC 35 NM
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Separation Size: 35-350nm
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Sample Volume: <1ml (Optimal: 500 µl)
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Volumetric Flow Rate at RT: >0.75ml/min
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Column Volume: 10ml
EVS SEC 70 NM
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Separation Size: 70-1000nm
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Sample Volume: <1ml (Optimal: 500 µl)
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Volumetric Flow Rate at RT: >0.75ml/min
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Column Volume: 10ml
Reusable columns and efficient packaging
Immunostep columns have been developed to facilitate its execution, and for this reason it comes with a box which easily transforms itself to a columns rack.
Figure 2. Graphic instructions to use column box as a rack, avoiding to get a specific column rack in the laboratory and facilitating the protocol.
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