Allows simultaneous determination of the percentage and the absolute number of cells per microliter of the sample in a single tube;
Identifies non-viable cells and non-specific background signal;
Quick and easy identification of double positive CD45 and CD34;
Isotype control (IgG1) included: highly reliable diagnostic results.
This kit allows the identification of CD34 + cells and link together their number with the identified known number of spheres discriminating between viable and non-viable cells and discarding false positives produced by non-specific staining of the isotype.
Stem Cell Kit is a kit designed for quantitative determination of hematopoietic stem cells (HSCs) CD34 positive due to the specificity of the following antibodies:
The anti-CD45 antibody recognizes type C tyrosine receptor protein phosphatase, a protein of 220 kD member of Leukocyte antigen family;
The anti-CD34 antibody recognizes the progenitor hematopoietic cell protein antigen CD34;
Additionally, the number of false positives caused by nonspecific staining can be detected with the use of CD34 isotype control (IgG1).
Stage 1: selection of microspheres population;
Stage 2: selection of viable cells population (section B);
Stage 3: using population from section B (viable cells), identify CD34+ cells;
Stage 4: Note the number and the percentage of cells CD34+.