This kit contains:
An R-phycoerythrin (R-PE) conjugated monoclonal antibody, which recognizes a platelet specific antigen;
Polyclonal antibodies conjugated whit fluoresce in isothiocyanate (FITC) against total human immunoglobulins, human IgA, IgM, and IgG;
A polyclonal antibody conjugated whit FITC as a negative control.
Immune Thrombocytopenic Purpura (ITP) is an autoimmune disorder characterised by a low platelet count and mucocutaneous bleeding. The autoantibodies are directed primarily to the platelet-specific receptors CD41a (GPIIb/IIIa) and CD42b (GPIb).
As a result, the sensitized platelets are rapidly cleared by the monocyte-macrophage cell systems. The determination of autoantibodies against thrombocytes allows differentiate immune from nonimmune thrombocytopenia.
Anti-human Igs specificity analysis shows a very low cross-reactivity (>7%) for all of them, making easy the correct identification of non-pathological sample.