Anti-SARS-Cov-2 ELISA kit is a highly sensitive and specific test developed by Immunostep under CSIC patent licence, for the precise detection of either IgG or IgA antibodies against the Mpro (3CLpro) Protein of SARS-Cov-2 virus.
12×8 wells microtiter strip plate coated with recombinant SARS-CoV-2 antigen in vacuum-sealed pouch. 2 microwell sealing foils.
120 µl of HRP-conjugated anti-human antibody (100X). Contains CMIT / MIT 3: 1 as a preservative.
50 ml wash buffer (20X). HEPES, NaCl and detergents. Contains Proclin300 as a preservative (<0.0014%).
12 ml Stop Solution (1X – Ready to use). 0.5M sulfuric acid (H2SO4)
12 ml of tetramethylbenzidine (TMB) chromogenic substrate. (1X – Ready to use)
25 ml of Antibody and Sample Diluent Buffer (1X – Ready to use). Buffer that minimizes non-specific binding, cross-reactivity and matrix interference, with blue dye. Contains CMIT / MIT 3: 1 as a preservative.
In an attempt to increase the diagnosis possibilities of COVID-19 patients, this assay was developed for the detection of specific antibodies against one of the 16 non-structural proteins, the main viral protease (Mpro or 3CLpro), which plays a critical role in viral replication.
The Mpro protease plays a vital role in processing the polyproteins that are translated from the viral RNA. Although this protein is not exposed in the viral particle, Mpro is elicited after viral infection. Like other b-coronaviruses, SARS-CoV-2 is a positive-sense RNA virus that expresses multiple proteins as a single polypeptide chain, and Mpro cleaves the polyprotein to release mature proteins for the virus. Inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering from the COVID-19 infection.
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CE/IVD kits are only suitable for clinical use in European Union and countries with EU conformity recognition agreements. Manufactured in Spain by Immunostep under CSIC Patent Licence.
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