Lyophilized Exosome Standards 

The purest lyophilized standards from human biofluids and different cell culture media

Lyophilization is a useful technique that can be used for long-term stable preservation of exosomes. Besides, lyophilized exosomes can be used as control standards for FACS, WB, ELISA and other applications, as well as quantitative calibration standards for foreign and domestic exosomes in biological samples. 

Immunostep Lyophilized Exosome Standards can be used as control for multiple applications, as calibration standards for biomarkers from biological samples and for immunocapture performance evaluation.


High pure exosomes improving performance over competitors

Purity validated by WB, NTA, Flow Cytometry and functional analysis In Vitro

Tested and validated for regenerative medicine, skin, dermatological and pharma companies

The purest Lyophilized Exosome Standards

Lyophilized Exosome Standards are easy to transport and storage and are stable for over 2 years at 2°C to 8°C.  Immunostep provides the highest quality Lyophilized Exosome Standards isolated from a variety of biological sources, including cell culture supernatant, human plasma, serum, urine, and saliva. Exosomes are isolated by differential ultracentrifugation.

Biofluids or Cell Culture Media

Sample Collection

Ultracentrifugal Purification

Lyophilization and Quantification

Guaranteed performance validated by WB, NTA, Flow Cytometry and functional analysis In Vitro

All exosome standard batches are validated using FCM, WB and NTA Analysis. Additionally, in order to compare the effects of lyophilization process we compare all lyophilized batches with respect to fresh exosomes stored at -20ºC. Dynamic range of fresh and lyophilized PC3 exosomes are analysed by Flow Cytometry.

Figure 1: Relationship between background noise and specific signal at different exosome concentrations. Exosomes were captured by CD63+ (Clone TEA3/18) Capture Beads and subsequently detected by Anti-CD9PE (Clone VJ1/20).

Video 1: Shows the exosomes characterization by Nanoparticle Tracking Analysis (NTA).

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Batch validation: High Quality and Pure Standards

For its validation, we carry out an exosomes analysis and comparative of fresh and lyophilized plasma exosomes for particle size and concentration by NTA, NanoSight LM10 HSB. This analysis is carried out with 1 μl of purified exosomes diluted in 999 μL of HEPES buffer (dilution1:1000). The purified exosomes show a size distribution profiles, with peak diameters from 50 –150 nm and concentrations about 1×10^12 exosomes/ml. Additionally, fresh and lyophilized MCF-7 exosome batches are analysed and compared by WB in native conditions for exosomal markers, by anti-CD9 (Clone VJ1/20) and anti-CD63 (Clon TEA3/18) antibodies at a 1:1000 dilution (0,1 mg/ml).


Kit NameKit Description
MSCs-derived Exosomes

Adipose-derived Mesenchymal stem/stromal cells (MSCs) derived exosomes | ExoMSC |

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Exosomes from PC-3

Human metastatic prostate cancer cell line | ExoPC3 | 0,1 mg

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Exosomes from HT-29

Human metastatic colon cancer cell line | ExoHT29 | 0,1 mg

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Exosomes from MCF-7

Human breast cancer cell line | ExoMCF7 | 0,1 mg

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Exosomes from Serum

human serum | ExoSERUM | 0,1 mg

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Exosomes from A-375

Human malignant melanoma cell line | ExoA375 | 0,1 mg

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Exosomes from RPMI

human myeloma cell line (RPM18) | ExoRPMI | 0,1 mg

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Exosomes from CaCo2

human colon cancer cell line | ExoCaCo2 | 0,1 mg

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Exosomes from A-549

Human lung cancer cell line | EXOA549 | 0,1 mg

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Exosomes from PANC-1

Human pancreas cancer cell line | EXOPANC1 | 0,1 mg

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