Protein Services

IMMUNOSTEP offers a wide range of services for the production and processing of recombinant proteins, from different protein expression platforms to a number of other protein services, such as the design of assays based on enzymes and antibodies, and customised processing and fabrication, for a great variety of applications and research areas. 

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Protein Expression and Purification

From a gene sequence, glycerol or plasmid, Immunostep carries out its recombinant protein production project. Moreover, we offer a recombinant protein characterisation service: 

Expression in mammalian cells

Transient transfection. Optimised expression and detection (293T, CHO and HEK cells). Post-translational modifications. Excellent method for the production of bioactive proteins. 

Bacterial Expression

Optimised expression and detection (E.coli). Rapid expression and easily scalable. 

Different techniques used for the purification of recombinant proteins: Ion exchange chromatography, affinity and molecular exclusion chromatography, or gel filtration chromatography. 

  • Tags used: 6xHis, GST, FLAG.

  • Tag removal by means of proteases: Thrombin, PreScission, Factor Xa.

Protein characterization

  • Electrophoresis in polyacrylamide gels with SDS and Coomassie staining.

  • Determination of protein concentration

  • Western blot

  • Absorption spectrum

  • HPLC

  • Size exclusion chromatography

Our service department has completed more than 100 projects involving the production of recombinant proteins for their use in functional assays, as antigens in the development of antibodies, structural analysis, and many other applications related to the production and characterisation of biomarkers and proteins of interest in areas such as diagnosis and therapy targets.

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Lyophilization Services   

Aiming at increasing the stability and useful life of proteins, and reducing freight charges, Immunostep offers customised protein lyophilisation services.

Our extensive experience minimises the risks of loss of activity or protein aggregation that may occur during lyophilisation by selecting the excipients and stabilisers for the process and adjusting the conditions of the lyophilisation. 

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LOCATION:

Centro Investigación del Cáncer (C.I.C) Avda. Universidad de Coimbra, s/n Campus Miguel de Unamuno 37007 Salamanca

+34 923 294 827 

info@immunostep.com

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