HeMoStep Kit:
The first tool to successfully detect and quantify blood contamination in cerebrospinal fluid
High sensitivity, accuracy, and reliability in just 55 minutes.
Welcome to the future of CSF testing! We are thrilled to introduce our groundbreaking method that will revolutionize how you DETECT and QUANTIFY blood contamination in cerebrospinal fluid samples.
With relative frequency, lumbar puncture procedure is complicated by the contamination of CSF with blood cells as result of needle trauma, also known as traumatic tap.
No more confusing diagnoses and analysis complications due to the presence of blood in Cerebrospinal Fluid samples thanks to the HemoStep Kit.
In addition, FlowStep software, developed for use with the kit, has proven to be an automated, efficient and accurate tool for the quantification of blood contamination in cerebrospinal fluid (CSF).
New high-performance method for evaluation of CSF contamination by Flow Cytometry
Data Analysis to extract and interpret assay results
Advantages
Principle of Method
The new method is based on a sandwich bead-based assay that enable the Hb quantification and correlated with the degree of contamination in RBC/µl.
Improved method by flow cytometry
The developed method allows the determination CSF peripheral blood contamination in a <1h protocol circumventing the problems arising from CSF cytotoxicity and minimizes sample usage, which is particularly important in paucicellular samples, by using as sample the supernatant of RBC lysis that is usually discarded.
Quantification of visibly undetectable contaminations
Expected values of contamination in [RBC/µ] (n=115).
High analytical sensitivity – LoD: [3,15 ng/ml]
The analytical sensitivity of the kit allows it to detect contaminations lower that 1 RBC/µ.
Correct classification between traumatic and non-traumatic LP samples with great accuracy
Expected values of contamination in [Hb ng/ml] in traumatic lumbar puncture (n=34).
Agreement between methods
High positive linear correlation (r=0.9) with respect to other methods, like granulocyte or RBC count (n=115).
Compatible with stabilized samples
Comparative between same sample stabilized with Transfix (1:20) and without stabilization (fresh).
Comparison of the estimated number of pathological cells by phenotype vs Hb kit in a group (n=13) of infiltrated samples
Samples in which the pathological cells identified could derive from contamintaion, resulting in a false positive for infiltration.
What this kit includes?
Capture Beads
5ml of Capture Beads coated with an Hb-specific monoclonal antibody.
Wash Buffer
25ml wash buffer (10X).
Control +
Positive control. 5 vials of lyophilised RBCs lysate.
Calibration Beads
1ml of calibration beads coated with two different concentrations of Hb.
Standard
Standard of known concentration. 1 vial of lyophilised RBCs lysate with known [Hb] concentrations.
Detector Antibody
1,2 ml fluorescently conjugated (PE) detector antibody (10μl/test).
FlowStep, the new automation tool for HeMoStep
The FlowStep software, designed specifically for integration with the HeMoStep kit, has proven to be an automated, efficient and accurate solution for the quantification of hematic contamination in cerebrospinal fluid (CSF).
It has a very high correlation with the manual method (r^2= 0.999), optimising reproducibility and decreasing dependence on highly specialised operators.
The implementation of advanced algorithms and automated report generation enables rapid and standardised analysis, increasing efficiency and improving the consistency of results in clinical laboratories.
For more information or to purchase, please contact us.