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Impact of data preprocessing on advanced cytometry results

Posted in Immunology.

Advanced flow cytometry has become an indispensable tool in biomedical research, immunology, and clinical biotechnology, enabling the characterization of cellular populations with unprecedented resolution. However,...

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Loading of non-coding nucleic acids in exosomes

Loading of non-coding nucleic acids in exosomes: microRNA, lncRNA, circRNA, and their functional significance

Posted in Exosomes.

In recent years, extracellular vesicles (EVs)—particularly exosomes—have emerged as key players in cellular communication. These nanosized vesicles released by cells act as carriers of biological information,...

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Exosomes heterogeneity

Exosome heterogeneity: functional subpopulations and their high-level characterization

Posted in Exosomes.

For years, exosomes were regarded as relatively homogeneous extracellular vesicles (EVs), simple carriers of biomolecules. However, advances in single-vesicle analysis have revealed a remarkable heterogeneity,...

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Exosomes as Advanced Modulators of the Immune System: Mechanisms and Therapeutic Opportunities

Posted in Exosomes.

Exosomes, a subset of extracellular vesicles (EVs) ranging from 30–150 nm, have emerged as active participants in immune system regulation. Beyond serving as passive carriers of molecular cargo, these...

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Optimisation of incubation and shaking protocols

Posted in Immunology.

Optimising incubation and agitation protocols is one of the fundamental pillars for ensuring reproducibility and sensitivity in cell-based assays. Often, small variations in time, temperature or movement...

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Calibration beads and their role in standardising clinical trials

Posted in Immunology.

In the field of advanced clinical research, the reproducibility and comparability of data between different laboratories and research centres is one of the most critical challenges. Variability in instrumentation,...

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Calibration beads

Optimisation of Multiplex Assays for Cell Therapies

Posted in Immunology.

The growing field of advanced cell therapies, including CAR-T and modified NK cells, demands comprehensive phenotypic and functional characterisation. In this context, multiplex assays have become essential...

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Fluorescent streptavidin dextrans

Fluorescent Streptavidin Dextrans: Innovation in Flow Cytometry

Posted in Immunology.

In the field of immunology, precision in cell detection and analysis is essential. Fluorescent Streptavidin Dextrans from Immunostep offer an advanced solution to enhance signal detection in flow cytometry...

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CD19 CAR-T in ALL

CD19 CAR-T in Acute Lymphoblastic Leukaemia

Posted in Immunology.

CD19-targeted CAR-T cells have transformed the management of acute lymphoblastic leukaemia (ALL), especially in refractory or relapsed paediatric and young adult patients. Accumulated clinical experience...

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Exosomes and CRISPR: Targeted gene editing in cancer and fibrosis

Posted in Exosomes.

Exosomes, small extracellular vesicles of 30–150 nm, have moved beyond being simple mediators of intercellular communication to become next-generation therapeutic vehicles. One of the most innovative...

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