Blog | Immunostep Biotech

The Molecular Language of Immune Exosomes: Is There a Cellular Vesicle Code?

Publicado en Exosomas.

For decades, cellular communication was primarily understood as an exchange of soluble molecules, receptors, and biochemical signals. However, in recent years, a new player has emerged in this complex...

Continuar leyendo

Compensation vs Unmixing in flow cytometry: key differences explained

Publicado en Immunología.

In multicolor flow cytometry, correcting spectral overlap between fluorochromes is a critical step to ensure data quality. In this context, the two main approaches — classical compensation and spectral...

Continuar leyendo

Exosomes and immune plasticity: how tumors reshape immune cell identity

Publicado en Exosomas.

The immune system is often described as a highly specialized defense network designed to recognize and eliminate abnormal cells. However, recent advances in tumor immunology have revealed that immune...

Continuar leyendo

How tooptimize panels with more than 18 colors without losing biological resolution

Publicado en Immunología.

The evolution of high-parameter flow cytometry has enabled the development of increasingly complex panels capable of characterizing extremely rare cell populations with unprecedented depth. However,...

Continuar leyendo

The role of exosomes in immunosuppresion within the tumor microenvironment

Publicado en Exosomas.

Cancer does not progress in isolation. On the contrary, it builds around itself a complex ecosystem in which tumor, stromal, and immune cells constantly interact. Within this context, exosomes have...

Continuar leyendo

How to assess T cell exhaustion by flow cytometry

Publicado en Immunología.

T cell exhaustion has become one of the most relevant concepts in modern immunology, particularly in the context of cancer, chronic infections, and cellular therapies such as CAR-T. As our understanding...

Continuar leyendo

Single-EV Analysis: Where Is the Field Heading?

Publicado en Exosomas.

The analysis of extracellular vesicles at the single-particle level (single-EV analysis) has rapidly evolved from a technological ambition into a scientific necessity. As the extracellular vesicle field...

Continuar leyendo

Annexin V is not enough: distinguishing apoptosis, necroptosis, and pyroptosis in flow cytometry

Publicado en Immunología.

In flow cytometry, the use of Annexin V has become one of the most widely adopted approaches for the analysis of cell death, particularly in the study of apoptosis. Its methodological basis, centered...

Continuar leyendo

HLA-DR as a dynamic biomarker: limitations and new interpretations in clinical immunology

Publicado en Immunología.

The human immune system is, by definition, dynamic. However, for many years clinical immunology has attempted to describe it using static biomarkers, single time-point measurements intended to summarize...

Continuar leyendo

Off-the-Shelf Cell Therapies: Current Status and Challenges

Publicado en Immunología.

Cell therapies have transformed immuno-oncology and regenerative medicine, with autologous CAR-T therapies being the most recognized for their clinical efficacy. However, the need for fast, scalable,...

Continuar leyendo