Immunology Archives | Immunostep Biotech

Flow cytometry and spatial biology: complementary technologies shaping the future of biomedical research

Understanding how cells behave has always been one of the fundamental goals of biomedical research. For decades, flow cytometry has been one of the most powerful techniques for identifying, quantifying,...

Does Manual Gating Still Make Sense in 2026?

Posted in Immunology.

Flow cytometry has evolved at an extraordinary pace over the last decade. What was once considered a complex panel of eight or ten colors has given way to experiments capable of analyzing more than...

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information?

Posted in Immunology.

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information? For many years, autofluorescence has been regarded as one of the major challenges in flow cytometry. High background signals,...

Compensation vs Unmixing in flow cytometry: key differences explained

Posted in Immunology.

In multicolor flow cytometry, correcting spectral overlap between fluorochromes is a critical step to ensure data quality. In this context, the two main approaches — classical compensation and spectral...

How tooptimize panels with more than 18 colors without losing biological resolution

Posted in Immunology.

The evolution of high-parameter flow cytometry has enabled the development of increasingly complex panels capable of characterizing extremely rare cell populations with unprecedented depth. However,...

How to assess T cell exhaustion by flow cytometry

Posted in Immunology.

T cell exhaustion has become one of the most relevant concepts in modern immunology, particularly in the context of cancer, chronic infections, and cellular therapies such as CAR-T. As our understanding...

Annexin V is not enough: distinguishing apoptosis, necroptosis, and pyroptosis in flow cytometry

Posted in Immunology.

In flow cytometry, the use of Annexin V has become one of the most widely adopted approaches for the analysis of cell death, particularly in the study of apoptosis. Its methodological basis, centered...

HLA-DR as a dynamic biomarker: limitations and new interpretations in clinical immunology

Posted in Immunology.

The human immune system is, by definition, dynamic. However, for many years clinical immunology has attempted to describe it using static biomarkers, single time-point measurements intended to summarize...

Off-the-Shelf Cell Therapies: Current Status and Challenges

Posted in Immunology.

Cell therapies have transformed immuno-oncology and regenerative medicine, with autologous CAR-T therapies being the most recognized for their clinical efficacy. However, the need for fast, scalable,...

The tumour microenvironment and NK cell suppression: key mechanisms and immunosuppressive

Posted in Immunology.

The tumour microenvironment (TME) constitutes a highly dynamic ecosystem in which tumour, stromal and immune cells interact in complex ways. In this context, NK (Natural Killer) cells—essential components...

Flow cytometry and spatial biology: complementary technologies shaping the future of biomedical research

Does Manual Gating Still Make Sense in 2026?

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information?

Compensation vs Unmixing in flow cytometry: key differences explained

How tooptimize panels with more than 18 colors without losing biological resolution

How to assess T cell exhaustion by flow cytometry

Annexin V is not enough: distinguishing apoptosis, necroptosis, and pyroptosis in flow cytometry

HLA-DR as a dynamic biomarker: limitations and new interpretations in clinical immunology

Off-the-Shelf Cell Therapies: Current Status and Challenges

The tumour microenvironment and NK cell suppression: key mechanisms and immunosuppressive

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