This solution has been designed to elute the exosomes from the antibody-bead complex and allow downstream analysis of the immunopurified exosomes without any type of interference. This product is compatible with #ExoStep kits family and #Capture beads product range.
The biological characterization of exosomes requires in most cases the isolation of intact exosomes. In this sense, many methods have been developed for the isolation of exosomes from biological fluids, among which are ultracentrifugation, chromatography, filtration, polymer-bases precipitation and immunological separation2. As a rule, the use of immunological methods requires the use of a solid substrate, usually magnetic beads, coated with antibodies or ligands directed against specific antigens on the exosome surface, allowing their specific isolation. In this way, in some cases for downstream exosome analysis it is necessary to elute the exosomes from the antibody-bead complex, avoiding potential interference.