Immunostep Blog | Innovations in Clinical Research and Diagnostic Solutions

Flow cytometry and spatial biology: complementary technologies shaping the future of biomedical research

Understanding how cells behave has always been one of the fundamental goals of biomedical research. For decades, flow cytometry has been one of the most powerful techniques for identifying, quantifying,...

Does Manual Gating Still Make Sense in 2026?

Posted in Immunology.

Flow cytometry has evolved at an extraordinary pace over the last decade. What was once considered a complex panel of eight or ten colors has given way to experiments capable of analyzing more than...

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information?

Posted in Immunology.

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information? For many years, autofluorescence has been regarded as one of the major challenges in flow cytometry. High background signals,...

The Molecular Language of Immune Exosomes: Is There a Cellular Vesicle Code?

Posted in Exosomes.

For decades, cellular communication was primarily understood as an exchange of soluble molecules, receptors, and biochemical signals. However, in recent years, a new player has emerged in this complex...

Compensation vs Unmixing in flow cytometry: key differences explained

Posted in Immunology.

In multicolor flow cytometry, correcting spectral overlap between fluorochromes is a critical step to ensure data quality. In this context, the two main approaches — classical compensation and spectral...

Exosomes and immune plasticity: how tumors reshape immune cell identity

Posted in Exosomes.

The immune system is often described as a highly specialized defense network designed to recognize and eliminate abnormal cells. However, recent advances in tumor immunology have revealed that immune...

How tooptimize panels with more than 18 colors without losing biological resolution

Posted in Immunology.

The evolution of high-parameter flow cytometry has enabled the development of increasingly complex panels capable of characterizing extremely rare cell populations with unprecedented depth. However,...

The role of exosomes in immunosuppresion within the tumor microenvironment

Posted in Exosomes.

Cancer does not progress in isolation. On the contrary, it builds around itself a complex ecosystem in which tumor, stromal, and immune cells constantly interact. Within this context, exosomes have...

How to assess T cell exhaustion by flow cytometry

Posted in Immunology.

T cell exhaustion has become one of the most relevant concepts in modern immunology, particularly in the context of cancer, chronic infections, and cellular therapies such as CAR-T. As our understanding...

Single-EV Analysis: Where Is the Field Heading?

Posted in Exosomes.

The analysis of extracellular vesicles at the single-particle level (single-EV analysis) has rapidly evolved from a technological ambition into a scientific necessity. As the extracellular vesicle field...

Flow cytometry and spatial biology: complementary technologies shaping the future of biomedical research

Does Manual Gating Still Make Sense in 2026?

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information?

The Molecular Language of Immune Exosomes: Is There a Cellular Vesicle Code?

Compensation vs Unmixing in flow cytometry: key differences explained

Exosomes and immune plasticity: how tumors reshape immune cell identity

How tooptimize panels with more than 18 colors without losing biological resolution

The role of exosomes in immunosuppresion within the tumor microenvironment

How to assess T cell exhaustion by flow cytometry

Single-EV Analysis: Where Is the Field Heading?

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