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Human IgA occurs in multiple molecular forms (polymeric and monomeric) and two subclasses which show differential distribution between the mucosal and circulatory compartments of the immune system. In humans, 85–90% of IgA antibodies are found in body secretions such us saliva, tears, breast milk; gastrointestinal mucous membranes, urogenital and respiratory tracts and prostatic fluid, among others. The other 10-15% of IgA antibodies are found in blood as monomeric, IgA1, and polymeric forms IgA2. IgA1 is present 5 times more than IgA2.
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Tested Applications | FCM (Quality tested), ELISA, WB, Multiplex, Immunocytochemistry-F. |
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Storage | Store in the dark at 2-8°C. |
Other names | IGHA1 |
Buffer | The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide (NaN3). |
Immunogen | Fc fragment of human IgA1 myeloma protein. |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. Typically 0,05 mg/ml. |
Immunostep’s anti-human IgA1, clone SAA1, is a monoclonal antibody intended for the identification of cells expressing IgA1 protein in peripheral blood using a compatible flow cytometer. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 10^6 cells or 100 ml of sample. Anti-IgA1 should be used with wash steps before reagent addition or with ficoll gradient separation to remove Immunoglobulins from serum.
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