FlowBoScan
PRE-MIXED COMBINATION READY TO USE
ToBoScan
SINGLE USE TUBE AVOIDING PIPETTING ERRORS
Consensus markers and independent of the stage of monocyte maturation
*Hypothesis by van Dongen and Orfao
Monocytes are produced by the bone marrow from precursors. Monocytes circulate in the bloodstream for about one to three days and then typically migrate into tissues throughout the body where they differentiate into macrophages which have the function of phagocytosing bacteria and damaged tissue.
Therefore the vast majority of monocytes (90-95%) in human blood are CD14++/CD16-/dim “classical monocytes”, whereas macrophages in human tissues are generally CD14dim/CD16+/++. Interestingly, in human lymph most monocytes/macrophages (65-95%) have the “non-classical” CD14dim/CD16++ phenotype. This suggests that the small population (5-10%) of CD14dim/CD16++ “non-classical monocytes” in blood are most likely Tissue macrophages (TiMas), which have returned from their patrolling and scavenger tasks in the body tissues.
Different studies have identified changes in the absolute and relative numbers of circulating monocytes and TiMas in clinical conditions with significant tissue disruption, such as in case of inflammation, sepsis, autoimmune disease, and cáncer. Therefore, acurate detection and definition of blood monocyte & TiMa subset represent a novel tool for early diagnosis and treatment monitoring in oncology and tissue homeostasis.
Strategy Analysis
Accurate flowcytometric gating on CD300e and HLA-DR allows to select for all monocytes/macrophage populations in blood.
Protocol
Bulk lysing or Lyse / Stain / Wash (LSW) protocol recommended to increase monocyte and TiMa subset concentration. In order to reach a high sensitivity > 10 million total events must be acquired.