Immunostep Blog | Innovations in Clinical Research and Diagnostic Solutions

NK cells in clinical practice: towards the therapeutic standardisation of cellular immunotherapy

Natural killer (NK) cells have emerged as one of the most promising components of cellular immunotherapy, thanks to their unique ability to identify and eliminate tumour cells independently of classical...

Spreading error as an experimental variable: How to quantify it and use it to optimize cytometry panels

Posted in Immunology.

In high-dimensional flow cytometry, the concept of spreading error is often mentioned in passing. Most discussions focus on spillover, the classical overlap of fluorochrome emission spectra, but spreading...

Phenotypic stability in multiparametric cytometry: The hidden challenge of longitudinal studies

Posted in Immunology.

The promise of high-dimensional cytometry High-dimensional multiparametric flow cytometry has become a cornerstone in translational immunology, biomarker discovery, and clinical trials. It allows researchers...

Real limitations of UMAP and t-SNE in flow cytometry: When dimensionality reduction distorts biology

Posted in Immunology.

Introduction: The promise and the risk of dimensionality reduction High-dimensional flow cytometry has dramatically transformed our ability to explore the complexity of the immune system, allowing researchers...

Flow cytometric detection of CAR-T cells: why two laboratories measure “the same thing” and obtain different results

Posted in Immunology.

Flow cytometry-based detection of CAR-T cells is now a cornerstone in immunotherapy research, translational studies, and clinical monitoring. It is routinely used to assess CAR-T expansion, persistence,...

Imaging Flow Cytometry (IFC) for Morphofunctional Analysis: Immunological Synapses and Cytotoxic Degranulation in CAR-T Cells

Posted in Immunology.

Imaging Flow Cytometry (IFC) combines the best of two worlds: the morphological resolution of microscopy with the statistical robustness of traditional flow cytometry. This advanced technique enables...

NK cells in clinical practice: towards the therapeutic standardisation of cellular immunotherapy

Spreading error as an experimental variable: How to quantify it and use it to optimize cytometry panels

Phenotypic stability in multiparametric cytometry: The hidden challenge of longitudinal studies

Real limitations of UMAP and t-SNE in flow cytometry: When dimensionality reduction distorts biology

Flow cytometric detection of CAR-T cells: why two laboratories measure “the same thing” and obtain different results

Imaging Flow Cytometry (IFC) for Morphofunctional Analysis: Immunological Synapses and Cytotoxic Degranulation in CAR-T Cells

Advanced Panel Design Strategies in Multicolor Flow Cytometry: A Guide for Researchers

Advanced Multiplexing for Cytokine Quantification in Microvolumes: Technical Challenges and Validation in CRS Assays

Multiparametric Cytometry Assays for Studying Metabolic Pathways in Immune Cells: Implications for Cold Tumor Resistance

Standardization and Validation of Assays for Exosome Quantification: Advanced Methods and Reproducibility Challenges

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