Anti- Human HLA-B27/HLA-B7 (HLA-ABC-m3; BB7.1)

The monoclonal antibodies included in this reagent are directed against the HLA-B27 and HLA-B7 antigens, both of which are polymorphic surface proteins belonging to the human MHC class I family. These molecules are expressed on all nucleated cells and consist of a specific transmembrane alpha chain non-covalently associated with beta-2 microglobulin 1.

HLA-B27 plays a central role in antigen presentation to CD8⁺ T cells, particularly in the context of intracellular pathogens. It is strongly associated with spondyloarthropathies, especially ankylosing spondylitis, where over 90% of affected individuals express this antigen, compared to only 7% in the general population2. Its expression is routinely assessed in clinical immunology to support diagnosis and disease monitoring.

HLA-B7, another classical MHC class I molecule, also presents endogenous peptides to cytotoxic T cells and is involved in immune surveillance. It is one of the most frequently expressed HLA-B alleles in the Caucasian population and is commonly included in HLA typing panels for transplantation compatibility 3.
Both antigens are relevant in the field of immunopeptidomics, where antibodies targeting MHC class I molecules are used to isolate peptide-MHC complexes for mass spectrometry analysis. This approach enables the identification of tumor-associated or pathogen-derived peptides, contributing to the development of personalized immunotherapies3.