This antibody reacts with the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones.
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide (NaN3).
Immunogen
Human leucocytes.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration. Typically 0,05 mg/ml.
Recomended usage
CD3, clone 33-2A3, is a mAb intended for the identification/enumeration of human T cells subsets using flow cytometry. This reagent is effective for direct IF staining of human tissue for FCM analysis using ≤1 μg/10^6 cells.