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Immunoglobulin (Ig) G (IgG) is the predominant of five classes of Ig (IgG, IgA, IgM, IgE, and IgD). Igs differ in heavy chain structure and effector function. IgG1, the largest IgG subclass, represents ~ 60% of IgG and has a half-life of 21 days. Antibody responses to soluble protein and membrane antigens primarily induce IgG1, although polysaccharides and allergens also elicit IgG1 responses.
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Storage | Store in the dark at 2-8°C. |
Other names | IGHG1 |
Buffer | The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide (NaN3). |
Immunogen | Human IgG1 myeloma protein. |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. Typically 0,05 mg/ml. |
Immunostep’s anti-human IgG1, clone SAG1, is a monoclonal antibody intended for the identification of cells expressing IgG1 protein in peripheral blood using a compatible flow cytometer. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 10^6 cells or 100 ml of sample. Anti-IgG1 should be used with wash steps before reagent addition or with ficoll gradient separation to remove Immunoglobulins from serum.
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