Custom Exosome Services
Exosomes are small (~40‐150 nm) extracellular vesicles (EVs) released from all cell types and found in body fluids and cell culture supernatants. Exosomes are generated by fusion of a specialized endosome, the multivesicular body (MVB), with the plasma membrane. Exosomes have been proposed to provide means for intercellular exchange of macromolecules, allowing the transfer cellular information, contributing to intercellular communication in relevant biological processes.
Exosome Isolation and Purification
Immunostep has developed a series of protocols for the isolation and purification of high-quality exosomes derived from multiple sources including cell culture samples and all human proximal fluids, including plasma, urine, cerebrospinal fluid, amniotic fluid, bronchoalveolar lavage fluid, synovial fluid, sperm, saliva, malignant and pleural effusions of ascites and breast milk.
Characterization Methods
We have developed and implemented several characterization and validation methods, for both research and clinical purposes, that allow analyze purity and composition and to quantify exosomal cargo, which contains critical information that has proved to be tremendously promising for diagnostic, prognostic and therapeutic use in a variety of disease areas. These methods include:
Electronic microscopy: exosomes isolated by a capture bead
Fluorescence: activated cell sorting (FACS)
Nanoparticle tracking analysis (NTA).Size and particle concentration analysis report.
Exosome Characterization
Exosome characterization and analysis presents a tremendous clinical opportunity and specifically in the area of liquid biopsy. However, realizing the potential requires detecting and characterizing exosomes with high accuracy and reproducibility, which can be challenging for any project or research. Immunostep makes available to its clients all their experience and technical capabilities in the field of exosomes, to make the clinical use of these EVs a reality.
