Qualitative immunoenzymatic determination of specific IgG antibodies against Covid-19 based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. The 96-well ELISA plates are coated with the specific recombinant antigen (Spike Protein or S protein) to bind the antibodies present in human serum and plasma samples (K2-EDTA, K3-EDTA, Heparin-Li, Sodium Citrate). The intended use of the assay is to aid in the identification of individuals with an adaptive immune response against SARS-CoV-2, indicating a previous or recent infection and contributing, in combination with other tests (PCR or antigen), to determining the stage of the infection. Additionally, this test can be used in research, seroprevalence studies, epidemiological surveillance and immune response of different vaccines against SARS-CoV-2.