Immunología Archives | Immunostep Biotech

Imaging Flow Cytometry (IFC) for Morphofunctional Analysis: Immunological Synapses and Cytotoxic Degranulation in CAR-T Cells

Imaging Flow Cytometry (IFC) combines the best of two worlds: the morphological resolution of microscopy with the statistical robustness of traditional flow cytometry. This advanced technique enables...

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Advanced Panel Design Strategies in Multicolor Flow Cytometry: A Guide for Researchers

Publicado en Immunología.

Multicolor flow cytometry has become an essential tool in immunology, oncology, and advanced cell therapies. However, designing complex panels that allow simultaneous analysis of multiple markers without...

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Advanced Multiplexing for Cytokine Quantification in Microvolumes: Technical Challenges and Validation in CRS Assays

Publicado en Immunología.

Cytokine quantification is a cornerstone of immunology, oncology, and advanced cell therapies. In complex clinical scenarios such as cytokine release syndrome (CRS) associated with CAR-T therapies or...

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Multiparametric Cytometry Assays for Studying Metabolic Pathways in Immune Cells: Implications for Cold Tumor Resistance

Publicado en Immunología.

Cellular metabolism has emerged as a key factor in immune response and tumor progression. The ability of immune cells to adapt to hostile environments depends on the activation of critical metabolic...

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Mass Cytometry (CyTOF) and Single-Cell Transcriptomics: A New Dimension for Analyzing the Tumor Microenvironment

Publicado en Immunología.

Studying the tumor microenvironment (TME) has become a central focus of modern oncology research. The complexity of solid tumors lies not only in the cancer cells themselves but also in the intricate...

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Impact of data preprocessing on advanced cytometry results

Publicado en Immunología.

Advanced flow cytometry has become an indispensable tool in biomedical research, immunology, and clinical biotechnology, enabling the characterization of cellular populations with unprecedented resolution. However,...

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Optimisation of incubation and shaking protocols

Publicado en Immunología.

Optimising incubation and agitation protocols is one of the fundamental pillars for ensuring reproducibility and sensitivity in cell-based assays. Often, small variations in time, temperature or movement...

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Calibration beads and their role in standardising clinical trials

Publicado en Immunología.

In the field of advanced clinical research, the reproducibility and comparability of data between different laboratories and research centres is one of the most critical challenges. Variability in instrumentation,...

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Optimisation of Multiplex Assays for Cell Therapies

Publicado en Immunología.

The growing field of advanced cell therapies, including CAR-T and modified NK cells, demands comprehensive phenotypic and functional characterisation. In this context, multiplex assays have become essential...

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Fluorescent Streptavidin Dextrans: Innovation in Flow Cytometry

Publicado en Immunología.

In the field of immunology, precision in cell detection and analysis is essential. Fluorescent Streptavidin Dextrans from Immunostep offer an advanced solution to enhance signal detection in flow cytometry...

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Imaging Flow Cytometry (IFC) for Morphofunctional Analysis: Immunological Synapses and Cytotoxic Degranulation in CAR-T Cells

Advanced Panel Design Strategies in Multicolor Flow Cytometry: A Guide for Researchers

Advanced Multiplexing for Cytokine Quantification in Microvolumes: Technical Challenges and Validation in CRS Assays

Multiparametric Cytometry Assays for Studying Metabolic Pathways in Immune Cells: Implications for Cold Tumor Resistance

Mass Cytometry (CyTOF) and Single-Cell Transcriptomics: A New Dimension for Analyzing the Tumor Microenvironment

Impact of data preprocessing on advanced cytometry results

Optimisation of incubation and shaking protocols

Calibration beads and their role in standardising clinical trials

Optimisation of Multiplex Assays for Cell Therapies

Fluorescent Streptavidin Dextrans: Innovation in Flow Cytometry

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