Blog | Immunostep Biotech

Advanced Panel Design Strategies in Multicolor Flow Cytometry: A Guide for Researchers

Multicolor flow cytometry has become an essential tool in immunology, oncology, and advanced cell therapies. However, designing complex panels that allow simultaneous analysis of multiple markers without...

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Advanced Multiplexing for Cytokine Quantification in Microvolumes: Technical Challenges and Validation in CRS Assays

Publicado en Immunología.

Cytokine quantification is a cornerstone of immunology, oncology, and advanced cell therapies. In complex clinical scenarios such as cytokine release syndrome (CRS) associated with CAR-T therapies or...

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Multiparametric Cytometry Assays for Studying Metabolic Pathways in Immune Cells: Implications for Cold Tumor Resistance

Publicado en Immunología.

Cellular metabolism has emerged as a key factor in immune response and tumor progression. The ability of immune cells to adapt to hostile environments depends on the activation of critical metabolic...

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Standardization and Validation of Assays for Exosome Quantification: Advanced Methods and Reproducibility Challenges

Publicado en Exosomas.

The study of exosome quantification has grown exponentially in recent years. These extracellular vesicles have proven to be valuable tools as disease biomarkers and potential vehicles in cell and gene...

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Mass Cytometry (CyTOF) and Single-Cell Transcriptomics: A New Dimension for Analyzing the Tumor Microenvironment

Publicado en Immunología.

Studying the tumor microenvironment (TME) has become a central focus of modern oncology research. The complexity of solid tumors lies not only in the cancer cells themselves but also in the intricate...

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Impact of data preprocessing on advanced cytometry results

Publicado en Immunología.

Advanced flow cytometry has become an indispensable tool in biomedical research, immunology, and clinical biotechnology, enabling the characterization of cellular populations with unprecedented resolution. However,...

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Loading of non-coding nucleic acids in exosomes: microRNA, lncRNA, circRNA, and their functional significance

Publicado en Exosomas.

In recent years, extracellular vesicles (EVs)—particularly exosomes—have emerged as key players in cellular communication. These nanosized vesicles released by cells act as carriers of biological information,...

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Exosome heterogeneity: functional subpopulations and their high-level characterization

Publicado en Exosomas.

For years, exosomes were regarded as relatively homogeneous extracellular vesicles (EVs), simple carriers of biomolecules. However, advances in single-vesicle analysis have revealed a remarkable heterogeneity,...

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Exosomes as Advanced Modulators of the Immune System: Mechanisms and Therapeutic Opportunities

Publicado en Exosomas.

Exosomes, a subset of extracellular vesicles (EVs) ranging from 30–150 nm, have emerged as active participants in immune system regulation. Beyond serving as passive carriers of molecular cargo, these...

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Optimisation of incubation and shaking protocols

Publicado en Immunología.

Optimising incubation and agitation protocols is one of the fundamental pillars for ensuring reproducibility and sensitivity in cell-based assays. Often, small variations in time, temperature or movement...

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Advanced Panel Design Strategies in Multicolor Flow Cytometry: A Guide for Researchers

Advanced Multiplexing for Cytokine Quantification in Microvolumes: Technical Challenges and Validation in CRS Assays

Multiparametric Cytometry Assays for Studying Metabolic Pathways in Immune Cells: Implications for Cold Tumor Resistance

Standardization and Validation of Assays for Exosome Quantification: Advanced Methods and Reproducibility Challenges

Mass Cytometry (CyTOF) and Single-Cell Transcriptomics: A New Dimension for Analyzing the Tumor Microenvironment

Impact of data preprocessing on advanced cytometry results

Loading of non-coding nucleic acids in exosomes: microRNA, lncRNA, circRNA, and their functional significance

Exosome heterogeneity: functional subpopulations and their high-level characterization

Exosomes as Advanced Modulators of the Immune System: Mechanisms and Therapeutic Opportunities

Optimisation of incubation and shaking protocols

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