The analysis of dynamic cellular processes – such as apoptosis, cell activation or the release of extracellular vesicles (EVs) – requires tools that are accurate, sensitive and compatible with real-time acquisition technologies. At Immunostep, we provide validated reagents and kits that enable basic, clinical and industrial research laboratories to follow these events with a high level of resolution and reproducibility.
Below highlights how our products can address each of these processes:
Assessment of apoptosis in cultured cells or primary samples is essential for studies of toxicity, therapeutic response or fundamental cell biology. Rather than relying solely on end-point methods, our kits allow closer to real-time observation with multi-parametric flow cytometry.
Recommended product: INTRACELL Apoptosis kit
Allows simultaneous detection of active caspase-3 and other intracellular signals related to apoptosis. It is based on conjugated antibodies and buffers optimised for gentle permeabilisation, ideal for preserving cell integrity during kinetic analysis.
Featured applications:
-Drug-induced apoptosis assays.
-Study of mitochondrial and programmed cell death pathways
-Real-time cell model validation by cytometry
Activation of immune cells – such as T or B lymphocytes, monocytes or NK cells – leads to changes in surface marker expression, cytokine production and reprogramming of effector functions. Immunostep offers validated conjugated antibodies for multi-parametric monitoring of these events.
Recommended products: CD69-FITC, CD25-APC, HLA-DR-PE
Monoclonal antibodies against late early markers of cell activation. Compatible with ex vivo stimulation assays and with timed activation protocols for temporal analysis in cytometry. Main applications:
The release of exosomes and microvesicles reflects physiological or pathological states of cells. Their dynamic analysis requires specific tools for their detection, quantification and phenotypic characterisation.
Recommended product: ExoStep
System based on magnetic beads pre-loaded with anti-CD9, CD-63 and CD-81 antibodies, for the capture of exosomes and subsequent analysis by flow cytometry, compatible with monitoring of EV production in cell cultures under controlled experimental conditions.
Featured applications:
In addition to the standard products, Immunostep offers:
Our scientific team works closely with public and private laboratories to ensure that each assay is designed to provide reliable, reproducible and actionable results for experimental decision making.