What is Paroxysmal Nocturnal Haemoglobinuria and how to treat it?

Paroxysmal nocturnal haemoglobinuria (PNH) is a rare, acquired and potentially severe haematological disease characterised by red blood cell destruction, thrombosis and bone marrow dysfunction. Its early and accurate diagnosis is key to proper patient management and timely therapeutic decisions.

The challenge of diagnosing PNH

The diagnosis of PNH is challenging due to the heterogeneity of its clinical manifestations. Patients may present with chronic haemolysis and haemolytic crises to venous thrombosis and extreme fatigue. Flow cytometry is the gold standard technique for the detection of cells deficient in GPI (glycosylphosphatidylinositol), the key marker of the disease.

Immunostep HPN Diagnostic Kit: Accuracy and Reliability

Immunostep’s HPN diagnostic kit is an innovative tool designed for accurate detection of the disease by flow cytometry. This kit allows the identification and quantification of GPI-deficient cell populations in peripheral blood samples, providing highly sensitive and specific results.

Main features of the kit:

-High sensitivity and specificity: Uses monoclonal antibodies directed against specific antigens of GPI-deficient cells.

-Compatible with any flow cytometer: It adapts to standard laboratory equipment, facilitating its implementation without the need for additional technology.

-Optimised and rapid protocol: It allows obtaining reliable results in a few hours, favouring an early diagnosis.

-Accurate quantification of the clonal population: Provides detailed information on the size of the HPN clone, essential for the evaluation of the patient’s prognosis and evolution.

Benefits of using the Immunostep kit in the diagnosis of PNH

The use of this kit in clinical and research settings offers multiple benefits:

1. Early and accurate diagnosis: rapid identification of PNH allows initiation of appropriate treatment without delay, improving the prognosis of patients.
2. Monitoring of disease progression: It allows assessment of the evolution of the clonal population in patients undergoing treatment.
3. Ease of use and adaptability: Its compatibility with conventional flow cytometers makes it accessible to many laboratories.
4. Reduction of diagnostic errors: Minimises the possibility of false positives or negatives thanks to its high specificity.

Conclusion

Diagnosis of Paroxysmal Nocturnal Haemoglobinuria is essential to improve the quality of life of patients and to avoid serious complications. Immunostep’s PNH diagnostic kit represents an advanced, accurate and efficient solution for the detection of this rare disease, facilitating both the initial diagnosis and the clinical follow-up of patients. Its implementation in specialised laboratories contributes to a more effective and personalised management of PNH, offering high-tech tools at the service of healthcare.