This product is used as red blood cell (RBC) lysis buffer and is supplied as a 10X solution. It should be diluted to 1X in deionized water before using.
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Samples must be prepared as single cell suspension in an appropriate anticoagulant (EDTA is recommended) tube.
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
Uso recomendado
Immunostep’s RBC Lysis solution, is intended for the lysis of whole red blood cells. This reagent works properly with samples of 4-11 x 10^3 leukocytes per microliter, usually this is equivalent to 100 µl of normal human or mouse whole blood sample. For samples with high cell number, dilute it with PBS to obtain the correct concentration.