{"id":36738,"date":"2025-07-29T13:24:50","date_gmt":"2025-07-29T11:24:50","guid":{"rendered":"https:\/\/immunostep.com\/?p=36738"},"modified":"2025-07-29T13:24:50","modified_gmt":"2025-07-29T11:24:50","slug":"real-time-monitoring-of-dynamic-cellular-processes-apoptosis-activation-and-exosome-release","status":"publish","type":"post","link":"https:\/\/immunostep.com\/es\/2025\/07\/29\/real-time-monitoring-of-dynamic-cellular-processes-apoptosis-activation-and-exosome-release\/","title":{"rendered":"Real-time monitoring of dynamic cellular processes: apoptosis, activation and exosome release"},"content":{"rendered":"

Advanced solutions available from Immunostep <\/strong><\/h4>\n

The analysis of dynamic cellular processes – such as apoptosis, cell activation or the release of extracellular vesicles (EVs) – requires tools that are accurate, sensitive and compatible with real-time acquisition technologies. At Immunostep, we provide validated reagents and kits that enable basic, clinical and industrial research laboratories to follow these events with a high level of resolution and reproducibility.<\/p>\n

Below highlights how our products can address each of these processes:<\/p>\n

Apoptosis: intracellular characterisation by flow cytometry <\/em><\/strong><\/h3>\n

Assessment of apoptosis in cultured cells or primary samples is essential for studies of toxicity, therapeutic response or fundamental cell biology. Rather than relying solely on end-point methods, our kits allow closer to real-time observation with multi-parametric flow cytometry.<\/p>\n

Recommended product: INTRACELL Apoptosis kit<\/a><\/strong><\/p>\n

Allows simultaneous detection of active caspase-3 and other intracellular signals related to apoptosis. It is based on conjugated antibodies and buffers optimised for gentle permeabilisation, ideal for preserving cell integrity during kinetic analysis.<\/p>\n

Featured applications:<\/strong><\/p>\n

-Drug-induced apoptosis assays.<\/p>\n

-Study of mitochondrial and programmed cell death pathways<\/p>\n

-Real-time cell model validation by cytometry<\/p>\n

Cell activation: monitoring of functional markers <\/em><\/strong><\/h3>\n

Activation of immune cells – such as T or B lymphocytes, monocytes or NK cells – leads to changes in surface marker expression, cytokine production and reprogramming of effector functions. Immunostep offers validated conjugated antibodies for multi-parametric monitoring of these events.<\/p>\n

Recommended products: CD69-FITC, CD25-APC<\/a>, HLA-DR-PE<\/a><\/strong><\/p>\n

Monoclonal antibodies against late early markers of cell activation. Compatible with ex vivo stimulation assays and with timed activation protocols for temporal analysis in cytometry. Main applications:<\/p>\n