{"id":31791,"date":"2025-02-20T09:25:19","date_gmt":"2025-02-20T08:25:19","guid":{"rendered":"https:\/\/immunostep.com\/?p=31791"},"modified":"2025-02-20T09:25:19","modified_gmt":"2025-02-20T08:25:19","slug":"basostep-advanced-basophil-activation-test-for-allergy-diagnosis","status":"publish","type":"post","link":"https:\/\/immunostep.com\/es\/2025\/02\/20\/basostep-advanced-basophil-activation-test-for-allergy-diagnosis\/","title":{"rendered":"BasoStep: Advanced Basophil Activation Test for Allergy Diagnosis"},"content":{"rendered":"<p>In allergy diagnostics, flow cytometry has revolutionized the detection and study of allergic reactions. Among these innovations, Immunostep&#8217;s <strong>BasoStep kit<\/strong> plays a key role in evaluating IgE-mediated disorders by analyzing basophil activation.<\/p>\n<h3><strong>What is BasoStep? <\/strong><\/h3>\n<p>BasoStep is an in <strong>vitro diagnostic kit<\/strong> that detects <strong>basophil activation<\/strong> in heparinized whole blood samples. It identifies the <a href=\"https:\/\/immunostep.com\/product\/cd63\/\"><strong>CD63<\/strong><\/a> marker, a membrane protein expressed on the surface of activated basophils after an allergen blast.<\/p>\n<p>This test makes it possible to determine the response of basophils to different stimuli, providing a highly specific and sensitive alternative to traditional methods such as skin tests and measurement of specific IgE in serum.<\/p>\n<h3><strong>Why is it important to measure basophil activation? <\/strong><\/h3>\n<p>Basophils play a key role in immediate allergic responses. When an allergen comes into contact with specific <strong>IgE bound to basophil<\/strong> membrane receptors, cellular activation occurs, leading to the release of inflammatory mediators. During this process, the <strong>CD63 <\/strong>antigen is expressed, a marker that indicates cellular activation and thus the patient&#8217;s sensitivity to the allergen in question.<\/p>\n<h3><strong>Benefits of using BasoStep in allergy diagnosis <\/strong><\/h3>\n<p>The BasoStep test provides significant advantages in the evaluation of IgE-mediated allergies:<\/p>\n<p><strong>-In vitro test without risk to the patient:<\/strong> Unlike skin tests, BasoStep does not require direct patient exposure to the allergen, reducing the risk of adverse reactions.<\/p>\n<p><strong>-High sensitivity and specificity:<\/strong> Flow cytometry allows detection of low levels of basophilic activation with high accuracy.<\/p>\n<p><strong>-Rapid and reproducible results:<\/strong> BasoStep&#8217;s methodology delivers reliable results within a few hours.<\/p>\n<p><strong>-Applicable to different allergens:<\/strong> It enables the study of multiple sensitizations in a single sample.<\/p>\n<p><strong>-Useful for monitoring treatments:<\/strong> Allows to evaluate the evolution of the immune response in patients undergoing allergic immunotherapy.<\/p>\n<h3><strong>What does the BasoStep kit include? <\/strong><\/h3>\n<p>The BasoStep kit contains all the reagents for the evaluation of basophilic activation:<\/p>\n<p>-Calcium, heparin and IL-3 stimulation buffer.<\/p>\n<p>-Positive control with fMLP and anti-IgE.<\/p>\n<p>-Staining reagent with monoclonal antibodies CD63 FITC, CD203c PE, HLA-DR PerCP and CD123 APC.<\/p>\n<p>-Positive and negative controls in lyophilized tubes for ease of use and accuracy.<\/p>\n<h3><strong>BasoStep test procedure <\/strong><\/h3>\n<p>The BasoStep protocol follows an optimized workflow to ensure maximum reliability:<\/p>\n<ol>\n<li><strong> Sample collection:<\/strong> The process begins by using heparinized whole blood.<\/li>\n<li><strong> Basophil stimulation:\u00a0<\/strong>The sample undergoes exposure to different allergens to induce cell activation.<\/li>\n<li><strong> Staining with specific antibodies:<\/strong> Reagents are added to label the cells of interest.<\/li>\n<li><strong> Flow cytometry analysis:<\/strong> CD63 expression is evaluated to determine the degree of basophil activation.<\/li>\n<\/ol>\n<h3><strong>BasoStep clinical applications <\/strong><\/h3>\n<p>BasoStep is a useful tool for various applications in immunology and allergology:<\/p>\n<p><strong>-Diagnosis of IgE-mediated allergies:<\/strong> it allows to identify specific sensitizations to different allergens.<\/p>\n<p><strong>Assessment of cross-reactivity:<\/strong> It is useful to determine the response to similar structural allergens.<\/p>\n<p><strong>Monitoring of immunotherapy treatments:<\/strong> It facilitates the follow-up of patients under desensitizing treatment.<\/p>\n<p><strong>-Immunology and allergy research:<\/strong> Its accuracy makes it ideal for clinical and laboratory studies.<\/p>\n<h3><strong>Conclusion <\/strong><\/h3>\n<p>Our BasoStep kit represents a breakthrough in allergy diagnostics by providing a reliable, safe and highly specific tool for the assessment of basophilic activation. Thanks to its flow cytometry-based approach and CD63 antigen detection, BasoStep offers an innovative alternative to traditional tests, with no risk to the patient and highly reproducible results.<\/p>\n<p>For more information on BasoStep and its application in allergy diagnosis, please visit our website: <a href=\"http:\/\/www.immunostep.com\">www.immunostep.com<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>In allergy diagnostics, flow cytometry has revolutionized the detection and study of allergic reactions. Among these innovations, Immunostep&#8217;s BasoStep kit plays a key role in evaluating IgE-mediated disorders by analyzing basophil activation. What is BasoStep? BasoStep is an in vitro diagnostic kit that detects basophil activation in heparinized whole blood samples. It identifies the CD63 [&hellip;]<\/p>\n","protected":false},"author":225,"featured_media":29099,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[2033],"tags":[2214,2207,2213,2170,2154,2117,2160,2215],"class_list":["post-31791","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-immunology","tag-allergies","tag-antibodies","tag-basostep","tag-flow-cytometry","tag-immune-cells","tag-immunology","tag-immunotherapies","tag-treatments"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>BasoStep: Advanced Basophil Activation Test for Allergy Diagnosis | Immunostep Biotech<\/title>\n<meta name=\"description\" content=\"At Immunostep we have been innovating since 2001 to develop comprehensive products that contribute to improve.\" \/>\n<meta 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