Immunostep Blog | Innovations in Clinical Research and Diagnostic Solutions

Flow Cytometry for Minimal Residual Disease (MRD): Key Factors for Sensitive and Reliable Detection

Minimal residual disease (MRD) has become an important parameter in the monitoring of patients with hematological malignancies. Detecting a very small population of abnormal cells after treatment can...

Monocyte Subsets by Flow Cytometry: Classical, Intermediate and Non-Classical Monocytes

Posted in Immunology.

of Monocytes are a heterogeneous population of innate immune cells that play essential roles in inflammation, antimicrobial defense, tissue repair, and immune regulation. Although they were traditionally...

CD45 in Flow Cytometry: Why This Marker Is More Than a Leukocyte Identifier

Posted in Immunology.

In flow cytometry, CD45 is often described simply as the “leukocyte marker.” However, its analytical value goes far beyond identifying white blood cells. The intensity of CD45 expression, particularly...

How to Avoid Batch Effects in Multi-Day Flow Cytometry Experiments

Posted in Immunology.

One of the greatest challenges in flow cytometry is ensuring that data generated over several days remain directly comparable. Whether you are processing patient samples collected over weeks, conducting...

Why Are My Cell Populations Disappearing? Common Sample Preparation Mistakes in Flow Cytometry

Posted in Immunology.

One of the most frustrating experiences in flow cytometry is preparing a sample that appears perfectly acceptable under the microscope, only to discover during acquisition that an expected cell population...

Stain Index: The Flow Cytometry Parameter That Should Guide Your Fluorochrome Selection

Posted in Immunology.

When researchers begin designing a multicolor flow cytometry panel, one of the first questions that usually comes to mind is: Which fluorochrome is the brightest? For years, fluorochrome brightness...

Flow cytometry and spatial biology: complementary technologies shaping the future of biomedical research

Posted in Immunology.

Understanding how cells behave has always been one of the fundamental goals of biomedical research. For decades, flow cytometry has been one of the most powerful techniques for identifying, quantifying,...

Does Manual Gating Still Make Sense in 2026?

Posted in Immunology.

Flow cytometry has evolved at an extraordinary pace over the last decade. What was once considered a complex panel of eight or ten colors has given way to experiments capable of analyzing more than...

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information?

Posted in Immunology.

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information? For many years, autofluorescence has been regarded as one of the major challenges in flow cytometry. High background signals,...

The Molecular Language of Immune Exosomes: Is There a Cellular Vesicle Code?

Posted in Exosomes.

For decades, cellular communication was primarily understood as an exchange of soluble molecules, receptors, and biochemical signals. However, in recent years, a new player has emerged in this complex...

Flow Cytometry for Minimal Residual Disease (MRD): Key Factors for Sensitive and Reliable Detection

Monocyte Subsets by Flow Cytometry: Classical, Intermediate and Non-Classical Monocytes

CD45 in Flow Cytometry: Why This Marker Is More Than a Leukocyte Identifier

How to Avoid Batch Effects in Multi-Day Flow Cytometry Experiments

Why Are My Cell Populations Disappearing? Common Sample Preparation Mistakes in Flow Cytometry

Stain Index: The Flow Cytometry Parameter That Should Guide Your Fluorochrome Selection

Flow cytometry and spatial biology: complementary technologies shaping the future of biomedical research

Does Manual Gating Still Make Sense in 2026?

Autofluorescence in Flow Cytometry: Noise or Valuable Biological Information?

The Molecular Language of Immune Exosomes: Is There a Cellular Vesicle Code?

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